Human

PX301A pdf (datasheet)

100 μg

$190.00 BUY

SW1353 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The SW1353 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM TrisHCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

2.0 mg/ml

PX301

Supplied in SDS sample buffer containing 50 mM DTT.

Store at 2-8°C for 6 months of continuous use. Store in working aliquots at -80°C for up to 1 year.

Centrifuge vial before use. Lysate is ready to load on SDS -PAGE for Western blotting. 20 µg per lane is recommended for a mini gel. The optimal concentration should be determined for each specific application.

WB