Human Thymus Tissue Lysate

Species:

Human

Cat. No.:

PX471A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Human thymus tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with BioRad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Concentration:

2 mg/ml

Data PDF:

PX471

Formulation:

Thymus tissue lysate is supplied in SDS sample buffer containing 5% βmercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. The human thymus tissue lysate is ready to load on SDSPAGE for Western blotting. It is recommended to load 10 μg to 20 μg per lane for mini gel.

Application:

WB