Human tPA Active ELISA Kit




alteplase, plasminogen activator, tissue type, reteplase, t-plasminogen activator, tissue plasminogen activator (t-PA), T-PA



Cat. No.:

CSI19829A pdf (datasheet)


1 Plate


$705.00 BUY


The sensitive quantitative measurement of functionally active human tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The basal level of tPA in healthy humans was found to be between 0.2-2 IU/ml. The assay measures active tPA in the 0-1 IU/ml range. Samples giving human tPA levels above 1 IU/ml should be diluted in blocking buffer before use. Samples of human plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the diluent provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. This kit cross reacts with rat, pig, and dog tPA and does not cross react with mouse tPA.

Entrez Symbol:


Uniprot Name:

Tissue-type plasminogen activator

Uniprot ID:


Kit Plates:

96-well precoated plate


Store all reagents at 2-8°C.