Human tPA Active ELISA Kit

Molecule:

TPA

Synonyms:

alteplase, plasminogen activator, tissue type, reteplase, t-plasminogen activator, tissue plasminogen activator (t-PA), T-PA

Species:

Human

Cat. No.:

CSI19829A pdf (datasheet)

Quantity/Size:

1 Plate

Price:

$705.00 BUY

Description:

The sensitive quantitative measurement of functionally active human tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The basal level of tPA in healthy humans was found to be between 0.2-2 IU/ml. The assay measures active tPA in the 0-1 IU/ml range. Samples giving human tPA levels above 1 IU/ml should be diluted in blocking buffer before use. Samples of human plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the diluent provided in the kit. Serum and cell culture media at neutral pH may also be used. Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. This kit cross reacts with rat, pig, and dog tPA and does not cross react with mouse tPA.

Entrez Symbol:

PLAT

Uniprot Name:

Tissue-type plasminogen activator

Uniprot ID:

P00750

Kit Plates:

96-well precoated plate

Storage/Stability:

Store all reagents at 2-8°C.