Human U937 Cell Lysate

Species:

Human

Cat. No.:

PX332A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$190.00 BUY

Description:

Human U937 Cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4 + 1% Triton X-100 + 0.2% sodium deoxycholate + 0.2% sodium dodecylsulfate (SDS) + 1 mM sodium ethylenediaminetetraacetate + 1 mM phenylmethylsulfonyl fluoride + 5 µg/ml of aprotinin + 5 µg/ml of leupeptin). Cell debris was removed by centrifugation.

Concentration:

2 mg/ml

Data PDF:

PX332

Formulation:

Lysate in SDS sample buffer with 5% β-mercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. Ready to load on SDS-PAGE. 10-20 µg per lane is recommended for mini gel.

Application:

WB