Description:
Human WI-38 Cell lysate was prepared by homogenization in modified RIPA buffer (50
mM Tris-HCl, pH 7.4 + 1% Triton X-100 + 0.2% sodium deoxycholate + 0.2% sodium
dodecylsulfate (SDS) + 1 mM sodium ethylenediaminetetraacetate + 1 mM phenylmethylsulfonyl fluoride + 5 µg/ml of aprotinin + 5 µg/ml of leupeptin). Cell debris was removed
by centrifugation.
Formulation:
Lysate in SDS sample buffer with 5% β-mercaptoethanol.
Storage/Stability:
Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.
Application notes:
Centrifuge vial before use. Ready to load on SDS-PAGE. 10-20 µg per lane is recommended for mini gel.
