Monkey IFN-gamma ELISA Kit

Molecule:

IFN-gamma

Species:

Monkey

Cat. No.:

CKM001 pdf (datasheet)

Quantity/Size:

5 Plates

Price:

$870.00 BUY

Description:

Cell Science’s Monkey IFN-gamma ELISA assay is a simple and sensitive sandwich immunoassay for the determination of monkey IFN-gamma in biological fluids such as cell culture supernatant, plasma or serum. The assay utilizes a coating antibody specific for monkey IFN-gamma coated on the wells of a 96-well microtiter plate. The wells are washed and blocked. Standards and samples are added to the wells and any analyte present binds to the immobilized coating antibody. After washing off excess/unbound materials, the bound analyte is allowed to associate with a biotinylated detection antibody specific for monkey IFN-gamma. The wells are washed again and a streptavidin-HRP conjugate (SSP) is added to the antibody-antigen-antibody complex. After another wash, a chromogenic substrate (TMB) is introduced, which produces a blue-colored product of which the intensity is related to the amount of analyte in the sample. A sulfuric acid solution is added to stop the enzymatic reaction (changing the color to yellow) and OD is read at 450 nm.

Kit Plates:

5 x 96-well microtiter plates, uncoated

Storage/Stability:

Store all reagents at 2-8°C.