Mouse Adipose Tissue Lysate

Species:

Mouse

Cat. No.:

PX361A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$190.00 BUY

Description:

Mouse adipose tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4 + 1% Triton X-100 + 0.2% sodium deoxycholate + 0.2% sodium dodecylsulfate (SDS) + 1 mM sodium ethylenediaminetetraacetate + 1 mM phenylmethylsulfonyl fluoride + 5 µg/ml of aprotinin + 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl, pH 6.8 + 12.5% glycerol + 1% SDS + 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Concentration:

2.0 mg/ml

Data PDF:

PX361

Formulation:

Whole cell lysate in 1 x SDS sample buffer containing 5% β-mercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. Mouse adipose tissue lysate is ready to load on SDSPAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

Application:

WB