Mouse Albumin ELISA Kit

Molecule:

Albumin

Species:

Mouse

Cat. No.:

CS376B pdf (datasheet)

Quantity/Size:

5 kit

Price:

$2,455.00 BUY

Description:

Background: Albumin is a water-soluble protein with considerable structural stability, which makes up 60% of the total protein of plasma. It functions as a carrier of hormones, enzymes, fatty acids, metal ions and medicinal products.

Assay Principle: Mouse albumin will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, peroxidase labeled polyclonal anti-mouse albumin antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450 nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse albumin. Color development is proportional to the concentration of albumin in the samples.

Sensitivity: 0.09 ng/ml

Specificity: Mouse albumin (Does not distinguish IgG subclasses.)

Range: 0.2-200 ng/ml

Sample Type: Mouse serum, plasma, urine and other biological fluids. For research use only.

Cross-Reactivity: Pooled normal plasma from rat was assayed and slight cross-reactivity was observed. Pooled normal plasma from rabbit, dog, pig, sheep cynomolgus monkey and human was assayed and no significant cross-reactivity was observed.

Kit Contents

  • 96-well microtiter strip plate coated with anti-Mouse albumin antibody, blocked and dried on well surface
  • 10X Wash Buffer Concentrate
  • 5X Diluent
  • Mouse albumin standard, lyophilized
  • Anti-Mouse albumin primary antibody, concentrated polyclonal Ab
  • TMB substrate solution

Data PDF:

CS376B