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Mouse Anti-FLAG Tag (Clone FG4R) mAb

Cat. No.:

CSI20568B pdf (datasheet)

Quantity/Size:

100 µg

Price:

$235.00 BUY

Concentration:

1.0 mg/ml

Description:

Mouse Anti-DYKDDDDK/FLAG Tag Clone FG4R monoclonal antibody

Data PDF:

CSI20568

Specificity:

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Host Species:

Mouse

Immunogen:

DYKDDDDK (FLAG) synthetic peptide conjugated to KLH

Isotype:

IgG1

Clone:

FG4R

Formulation:

Liquid in 10 mM PBS, pH 7.2, with 0.05% sodium azide.

Purification:

Protein A affinity chromatography from mouse ascites fluid

Application:

DB , ELISA, IP , IS , WB

Application notes:

Western blot sensitivity (with ECL): 1-5 ng of DYKDDDDK-tagged fusion protein
Western Blot (with ECL): working dilution 1:1,000-3,000 incubate for 1 hour at RT.
Immunoprecipitation: working dilution 1:500-2,000.
Immunostaining: working dilution of 1:500-2,000

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Upon receipt, store at -20°C to -80°C for up to 1 year. Upon 1st thaw, centrifuge vial briefly to consolidate liquid. Recommended to prepare aliquots for long-term storage at -20°C to -80°C. For continuous use, an aliquot may be stored at 2-8°C for several days to weeks. Avoid repeated freeze-thaw cycles.