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Western blot sensitivity (with ECL): 1-5 ng of DYKDDDDK-tagged fusion proteinWestern Blot (with ECL): working dilution 1:1,000-3,000 incubate for 1 hour at RT.Immunoprecipitation: working dilution 1:500-2,000.Immunostaining: working dilution of 1:500-2,000
The optimal concentration should be determined by the user for each specific application.