Mouse Anti-GAPDH (Clone GA1R) mAb Loading Control

Synonyms:

Glyceraldehyde-3-phosphate dehydrogenase

Cat. No.:

CSI20571B pdf (datasheet)

Quantity/Size:

100 µg

Price:

$250.00 BUY

Concentration:

1.0 mg/mL

Description:

The monoclonal antibody recognizes multi-species GAPDH. GAPDH performs many mechanistically distinct functions. Primarily, the protein catalyzes an important energyyielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Additionally, it has been identified to have uracil DNA glycosylase activity in the nucleus, antimicrobial activity against E. coli, P. aeruginosa, and C. albicans, and in mice, a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophages.

Anti-GAPDH is used as a loading control monoclonal antibody.

Data PDF:

CSI20571

Specificity:

Recognizes native and denatured forms of GAPDH (approx. 37kDa) from E. coli, Sf9 insect cells, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken and hamster. GAPDH from other species may also be detectable.

Host Species:

Mouse

Immunogen:

Recombinant GAPDH

Isotype:

IgG1

Clone:

GA1R

Formulation:

Liquid in 10 mM PBS, pH 7.2, 0.5% sodium azide.

Purification:

Protein A affinity chromatography from mouse ascites fluid

Application:

DB , ELISA, IS , WB

Application notes:

Western blot (with ECL) : working dilution of 1:1,000-100,000

Immunostaining: working dilution of 1:500- 2,000.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Centrifuge briefly to maximize product recovery. Prepare working aliquots and store at 2 -8°C for up to 1 week or at -20°C to -80°C for up to one year. Avoid repeated freeze-thaw cycles.