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The monoclonal antibody recognizes multi-species GAPDH. GAPDH performs many mechanistically distinct functions. Primarily, the protein catalyzes an important energyyielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Additionally, it has been identified to have uracil DNA glycosylase activity in the nucleus, antimicrobial activity against E. coli, P. aeruginosa, and C. albicans, and in mice, a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophages.
Anti-GAPDH is used as a loading control monoclonal antibody.
Western blot (with ECL) : working dilution of 1:1,000-100,000
Immunostaining: working dilution of 1:500- 2,000.
The optimal concentration should be determined by the user for each specific application.