Mouse Anti-Human Apo-E, Very Low Density Lipoprotein (Clone 3D12) mAb

Molecule:

APOE

Synonyms:

Apolipoprotein E

Cat. No.:

MON5029 pdf (datasheet)

Quantity/Size:

0.5 mg

Price:

$655.00 BUY

Description:

The monoclonal antibody recognizes Very Low Density Lipoprotein (Apo-E), a major apoprotein of the chylomicron which binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents.

Entrez Symbol:

APOE

Uniprot Name:

Apolipoprotein E

Uniprot ID:

P02649

Data PDF:

MON5029

Specificity:

The antibody reacts with very low density lipoproteins (VLDL) of human plasma. It is specific for human APO-E. This antibody recognizes all alleles of APO-E. This antibody reacts as well with LDL as with VLDL.

Host Species:

Mouse

Isotype:

IgG1

Clone:

3D12

Formulation:

Lyophilized from 1 ml of a purified Ig solution with approximately 0.5 mg/ml of antibody. (Sufficient for staining of 100 frozen sections.)

Purification:

Ammonium Sulfate precipitation, DEAE-cellulose chromatography, gradient elution.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute with 1 ml sterile distilled water. Do not vortex. Add preservative if preferred.

Application:

ELISA, IHC

Application notes:

ELISA: suggested 1:1000
IHC-F: suggested 1:100
IHC-P: possible use, the optimal dilution must be determined by the user.

For depletion experiments the antibody should be used in a solid phase system.

Storage/Stability:

Store at 2-8°C.