Mouse Anti-Human CD28 Clone B-T3 PE mAb

Molecule:

CD28

Synonyms:

CD28, CD28 antigen, Tp44

Species:

Human

Cat. No.:

CDM173 pdf (datasheet)

Quantity/Size:

100 tests

Price:

$420.00 BUY

Concentration:

100 tests/ml

Description:

The mouse monoclonal antibody recognizes human CD28, a cell-surface protein essential for T-cell activation. CD28 provides T cells with a "second signal," or costimulation, which, along with T cell receptor (TCR) ligation, induces proliferation and survival, and cytokine production. TCR stimulation without co-stimulation results in anergy of the T cell, CD28 interacts with CD80 (B7.1) and CD86 (B7.2), found on antigen presenting cells (APCs). Product: Derived from a hybrid of Myeloma X63/AG.8653 x Balb/c spleen cells.

Entrez Symbol:

CD28

Uniprot Name:

T-cell-specific surface glycoprotein CD28

Uniprot ID:

P10747

Data PDF:

CDM173

Specificity:

Human | Recognizes B7/BB1 receptor (B7/BB1 R), a 44 kDa protein

Host Species:

Mouse

Immunogen:

U266 cell line

Isotype:

IgG2a

Clone:

B-T3

Conjugate:

PE

Formulation:

Lyophilized from a solution in phosphate-buffered saline with 1% BSA and 0.1% sodium azide.

Purification:

Ion exchange Chromatography

Reconstitution:

Reconstitute with 1 ml deionized water.

Application:

FC

Application notes:

Flow Cytometry: Use 10 μl to label 1x10E6 cells or 100 μl of whole blood.

Storage/Stability:

Stable at +2-8°C for six months after reconstitution. DO NOT FREEZE.