Mouse Anti-Human Desmin Clone D33 mAb

Molecule:

DES

Synonyms:

CSM1, CSM2, LGMD2R, intermediate filament protein

Species:

Human

Cat. No.:

MON3001-1 pdf (datasheet)

Quantity/Size:

1 ml

Price:

$510.00 BUY

Concentration:

~25 µg/mL

Description:

The monoclonal antibody recognizes human desmin, a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intra-cytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane.

Entrez Symbol:

DES

Data PDF:

MON3001

Specificity:

Highly reactive with human desmin. On immunoblots, only the 52 kDa desmin band is detected. The antibody stains muscle cells in human tissues.

Host Species:

Mouse

Immunogen:

Human Leiomyoma

Isotype:

IgG1

Clone:

D33

Conjugate:

Unconjugated

Formulation:

Supernatant diluted in 0.15 M PBS with 1% BSA and 0.1% sodium azide. 1 ml sufficient for 100-500 tests.

Application:

IF, IHC-F, WB

Application notes:

Western blot: a preparation of leiomyoma cells is recommended for positive control.

IHC/ IF: For staining muscle cells in human tissues dilute the antibody approximately 1:100, preferably in PBS Frozen sections show excellent staining in IHC/ IF, while staining is somewhat reduced on sections of formalin-fixed and paraffin-embedded tissue. Positive Controls: Skeletal muscle, rhabdomyosarcomas, and leiomyoma cells.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Stable at 2-8 °C for up to 1 year.