DES

CSM1, CSM2, LGMD2R, intermediate filament protein

Human

MON3001-5 pdf (datasheet)

5 ml

Please Inquire

~25 µg/mL

The monoclonal antibody recognizes human desmin, a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intra-cytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane.

DES

MON3001

Highly reactive with human desmin. On immunoblots, only the 52 kDa desmin band is detected. The antibody stains muscle cells in human tissues.

Mouse

Human Leiomyoma

IgG1

D33

Unconjugated

Supernatant diluted in 0.15 M PBS with 1% BSA and 0.1% sodium azide. 1 ml sufficient for 100-500 tests.

IF, IHC-F, WB

Western blot: a preparation of leiomyoma cells is recommended for positive control.

IHC/ IF: For staining muscle cells in human tissues dilute the antibody approximately 1:100, preferably in PBS Frozen sections show excellent staining in IHC/ IF, while staining is somewhat reduced on sections of formalin-fixed and paraffin-embedded tissue. Positive Controls: Skeletal muscle, rhabdomyosarcomas, and leiomyoma cells.

The optimal concentration should be determined by the user for each specific application.

Stable at 2-8 °C for up to 1 year.