Mouse Anti-Human IL-11 mAb

Molecule:

IL-11

Synonyms:

IL-11, Adipogenesis Inhibitory Factor, AGIF, Oprelvekin

Species:

Human

Cat. No.:

CMI247 pdf (datasheet)

Quantity/Size:

500 μg

Price:

$685.00 BUY

Description:

Interleukin-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. IL-11 exerts its biological activities through binding to a specific high-affinity receptor complex consisting of an IL-11 receptor chain and gp130.

Entrez Symbol:

IL11

Uniprot Name:

Interleukin-11

Uniprot ID:

P20809

Data PDF:

CMI247

Specificity:

Recognizes human IL-11

Host Species:

Mouse

Immunogen:

Recombinant human IL-11

Isotype:

IgG1

Formulation:

Lyophilized from PBS

Purification:

Protein G affinity chromatography from ascites fluid.

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1.0 mg/ml. Do not vortex

Application:

ELISA, IS , Neutralizing

Application notes:

ELISA: when used at a concentration of 8-9 µg/ml (assuming 100 µl/well antibody solution), in conjunction with biotinylated antigen affinity purified anti-human IL11 as the detection antibody at a concentration of at least 2 µg/ml, allows the detection of at least 0.2 ng/well.

Neutralization: requires a concentration of 2.4-3.6 µg/ml to yield one-half maximal inhibition [ND50] of the biological activity of human IL11 (5.0 ng/ml).

Immunostaining: This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 μg/ml overnight at 2-8˚C This was followed by a fluorophore conjugated secondary antibody.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store lyophilized antibody at -20°C to -80°C for up to 1 year. Reconstituted antibody is stable at 2-8°C for at least six weeks or stored in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.