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Mouse Anti-Human IL-12 p35 + p70 Clone B-T21 mAb

Molecule:

IL-12

Synonyms:

CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B

Species:

Human

Cat. No.:

CDM271 pdf (datasheet)

Quantity/Size:

500 μg

Price:

$650.00 BUY

Concentration:

1 mg/ml

Description:

Mouse Anti-Human IL12p70 Clone B-T21 Azide Free mAb

This antibody can be used as a Capture Antibody in a human IL-12 sandwich Immunoassay to detect human IL-12 in combination with biotinylated human IL-12 p70 Detection Antibody (Cat No CDM429).

Background: Interleukin 12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development.

Entrez Symbol:

IL12A | IL12B

Uniprot Name:

Interleukin-12 subunit alpha | Interleukin-12 subunit beta

Uniprot ID:

P29459 | P29460

Data PDF:

CDM271

Specificity:

Human | Recognizes both natural and recombinant human IL-12 p35 + p70.

Host Species:

Mouse

Immunogen:

Recombinant human IL-12

Isotype:

IgG1

Clone:

B-T21

Conjugate:

Unconjugated

Formulation:

Liquid in phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free.

Purification:

Ion exchange Chromatography

Application:

ELISA, ELISPOT, FC, FS

Application notes:

Blocks IL-12 induced proliferation on PHA and IL-2 activated lymphocytes.
Suitable for intra-cytoplasmic flow cytometry.
ELISA Capture Ab: 0.3-1 µg/ml ELISPOT Capture: 5-10 µg/ml

Storage/Stability:

Store at +2-8°C for 12 months. For longer storage, freeze aliquots.