Mouse Anti-Human IL-4 (Clone B-R14) mAb, Azide Free

Molecule:

IL-4

Synonyms:

Interleukin-4, B cell growth factor, BCGF-1, BCGF1, BSF-1, BSF1

Species:

Human

Cat. No.:

CDM104B pdf (datasheet)

Quantity/Size:

500 μg

Price:

$650.00 BUY

Concentration:

1 mg/ml

Description:

IL-4 is a lymphokine that co-stimulates the proliferation of activated B- and T-cells, augments the cytotoxic activity of lymphocytes and monocytes and enhances the functional activity of myeloid cells. Produced by mast cells, T-cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ cells into helper Th2 cells characterized by their cytokine secretion-profile that includes secretion of IL-4, IL-5, IL-6, IL-10 and IL-13 which favour a humoral immune response. In addition, IL-4 can inhibit the proliferation of TNF, IL-1 and IL-6 by macrophages IL-4 induces B-cell class switching to IgE and IgG1 isotypes, and up regulates MHC Class II production and CD23 expression.

Entrez Symbol:

IL4

Uniprot Name:

Interleukin-4

Uniprot ID:

P05112

Data PDF:

CDM104

Specificity:

Recognizes both native and recombinant human IL-4

Host Species:

Mouse

Immunogen:

Recombinant human IL-4

Isotype:

IgG1 Kappa light chain

Clone:

B-R14

Formulation:

Sterile-filtered PBS, carrier and preservative free.

Purification:

Ion exchange chromatography

Application:

ELISA, ELISPOT

Application notes:

ELISA: Use as Capture Antibody at 0.05-0.5 µg/ml, in a human IL-4 sandwich ELISA in combination with biotinylated human IL-4 Detection Antibody (Cat No CDM425).

ELISPOT: Use as Capture Antibody at 5-10 µg/ml for human IL-4.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store at 2-8°C for up to 1 year or in working aliquots at -20°C to -80oC. Avoid repeated freeze-thaw cycles.