Mouse Anti-Human IL-6 Clone 8H12 mAb

Molecule:

IL-6

Species:

Human

Cat. No.:

CMI302 pdf (datasheet)

Quantity/Size:

0.5 mg

Price:

$620.00 BUY

Concentration:

1 mg/ml

Description:

IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis

Data PDF:

CMI302

Purity:

>95% as determined by SDS-PAGE analysis.

Specificity:

Human IL-6

Host Species:

Mouse

Immunogen:

Purified recombinant human IL-6.

Isotype:

IgG1

Clone:

8H12

Formulation:

Sterile-filtered PBS, pH 7.2 containing 0.1% sodium azide

Purification:

Protein A/G affinity chromatography.

Application notes:

ELISA as a detection antibody
Western blot
Flow Cytometry to detect intracellular IL-6 levels
For flow cytometry, 0.1-0.5 µg 8H12 stains 106 human peripheral blood mononuclear cells stimulated with LPS.
The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store at 2-8°C for up to one month. For long term use, store in aliquots below -20°C. Avoid repeated freeze-thaw cycles.