Mouse Anti-Human LBP (Clone biG 412) mAb

Molecule:

LBP

Synonyms:

Lipopolysaccharide Binding Protein, LPS-binding protein, BPI fold containing family D, member 2, BPIFD2

Species:

Human

Cat. No.:

CML004 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$415.00 BUY

Concentration:

> 1 mg/ml (lot-specific) IgG content determined by mouse IgG ELISA prior to lyophilization.

Description:

LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).

This antibody directly influences the interaction of CD14-LPS-LBP. The binding site is at the C-terminal end of LBP.

Entrez Symbol:

LBP

Uniprot Name:

Lipopolysaccharide-binding protein

Uniprot ID:

P18428

Data PDF:

CML004

Host Species:

Mouse

Immunogen:

LBP knockout mouse was immunized with immunoaffinity purified recombinant human LBP

Isotype:

IgG1

Clone:

biG 412

Formulation:

Lyophilized from a concentrated protein solution containing PBS, pH 7.2. Content was determined by OD280 nm. No preservatives or stabilizer added.

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Reconstitute with sterile ultra-pure deionized water. This product can be diluted further with PBS or other buffers.

Application:

ELISA, FS , INH

Application notes:

ELISA: 1:5,000
LBP inhibition studies: 1:1000, Inhibits LPS binding to membrane bound CD14.
Not useful for Western Blot
The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Upon receipt, stored as supplied for up to 1 year at -20°C to -80°C Reconstituted antibody should be used immediately or stored in working aliquots at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% BSA). Avoid repeated freeze/thaw cycles.