Mouse Anti-Human Neurofilament (Clone 2F11) mAb

Molecule:

NF

Synonyms:

NFH, NFL, CMT1F, CMT2E

Species:

Human

Cat. No.:

MON3004-1 pdf (datasheet)

Quantity/Size:

1 ml

Price:

$510.00 BUY

Concentration:

~50 µg/mL (sufficient for 100-500 tests)

Description:

The monoclonal antibody recognizes human neurofilaments. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.

Uniprot Name:

Neurofilament heavy polypeptide

Uniprot ID:

P12036

Specificity:

This antibody stains the 70 kD (light) and 200 kD (heavy) polypeptides of neurofilament.

Host Species:

Mouse

Immunogen:

Neurofilament from human brain

Isotype:

IgG1

Clone:

2F11

Conjugate:

Unconjugated

Formulation:

1 mL supernatant with approximately 60 μg antibody/ml, 1% FCS, 10 mM sodium azide.

Application:

IHC, WB

Application notes:

The antibody stains neurons in sections of brain and other tissues. Frozen sections show excellent staining. On sections from formalin fixed and paraffin embedded tissue the staining is somewhat reduced.

IHC - frozen: dilute approximately 1:160, preferably in PBS
IHC - paraffin: dilute approximately 1:50.
WB: dilute 1:20 (lesser amounts of the antibody may be sufficient)

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store antibody at 2-8°C for up to 1 year.