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The antibody stains neurons in sections of brain and other tissues. Frozen sections show excellent staining. On sections from formalin fixed and paraffin embedded tissue the staining is somewhat reduced. IHC - frozen: dilute approximately 1:160, preferably in PBSIHC - paraffin: dilute approximately 1:50. WB: dilute 1:20 (lesser amounts of the antibody may be sufficient) The optimal concentration should be determined by the user for each specific application.