Mouse Anti-Human Perforin Clone B-D48 Biotin Detection mAb

Molecule:

PRF1

Synonyms:

P1, PFP, FLH2, PFN1, HPLH2

Species:

Human

Cat. No.:

CDM438 pdf (datasheet)

Quantity/Size:

100 µg

Price:

$650.00 BUY

Concentration:

0.1 mg / 1.0 ml

Description:

Perforin 1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood.

Entrez Symbol:

PRF1

Uniprot Name:

Perforin-1

Uniprot ID:

P14222

Data PDF:

CDM438

Specificity:

Recognizes human perforin, a 70 kDa protein

Host Species:

Mouse

Immunogen:

Native human perforin

Isotype:

IgG1

Clone:

B-D48

Conjugate:

Biotin

Formulation:

Liquid in PBS with 1% BSA and 0.09% Sodium Azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.

Purification:

Affinity chromatography

Application:

ELISA

Application notes:

ELISA Detection Antibody. This antibody can be used as a Detection Antibody in a human Perforin sandwich Immunoassay to detect human Perforin in combination with human Perforin Capture Antibody (Cat No CDM414). The suggested coating concentration range below should be optimized by each laboratory for each application.
ELISA / ELISPOT: 0.05-0.5 µg/ml

Storage/Stability:

Store at 2-8°C for 12 months. For longer storage, freeze aliquots at -20°C. Avoid repeated freeze-thaw cycles.