Mouse Anti-Human Perforin Clone B-E48 Azide Free Capture mAb

Molecule:

PRF1

Synonyms:

P1, PFP, FLH2, PFN1, HPLH2

Species:

Human

Cat. No.:

CDM414 pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,150.00 BUY

Concentration:

1.0 mg / 1.0 ml

Description:

Perforin 1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood.

Entrez Symbol:

PRF1

Uniprot Name:

Perforin-1

Uniprot ID:

P14222

Data PDF:

CDM414

Specificity:

Recognizes human perforin, a 70 kDa protein

Host Species:

Mouse

Immunogen:

Native human perforin

Isotype:

IgG2b

Clone:

B-E48

Conjugate:

Unconjugated

Formulation:

Sterile-filtered PBS, carrier and preservative free.

Purification:

Affinity chromatography

Application:

ELISA, ELISPOT

Application notes:

ELISA / ELISPOT Capture Antibody. This antibody can be used as a Capture Antibody in a human Perforin sandwich immunoassay to detect human Perforin in combination with biotinylated human Perforin Detection Antibody (Cat No CDM438). The suggested coating concentration range below should be optimized by each laboratory for each application.
ELISA: 0.5-2.5 µg/ml
ELISPOT: 5-10 µg/ml.

Storage/Stability:

Store at 2-8°C for 12 months. For longer storage, freeze aliquots at -20°C. Avoid repeated freeze-thaw cycles.