Mouse Anti-Human Perforin-1 (Clone B-E48) mAb, Azide Free

Molecule:

Perforin

Synonyms:

P1, Cytolysin, Lymphocyte pore-forming protein, PFP

Species:

Human

Cat. No.:

CDM090C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,150.00 BUY

Concentration:

1 mg/ml

Description:

Perforin 1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood.

Entrez Symbol:

PRF1

Uniprot Name:

Perforin-1

Uniprot ID:

P14222

Data PDF:

CDM090

Specificity:

Recognizes human Perforin, a 70 kDa protein

Host Species:

Mouse

Immunogen:

Native human perforin

Isotype:

IgG2b Kappa light chain

Clone:

B-E48

Formulation:

Sterile-filtered PBS, carrier and preservative free.

Purification:

Ion exchange chromatography

Application:

ELISA, ELISPOT

Application notes:

ELISA: Use as a Capture Antibody at 0.5-2.5 µg/ml in a human Perforin sandwich ELISA in combination with biotinylated human Perforin Detection Antibody (Cat No CDM438).

Optimal coating concentration should be determined by the user.

Storage/Stability:

Stable at +2-8°C for 12 months. DO NOT FREEZE.