Mouse Anti-Human TIMP1 Clone #4A16 mAb

Molecule:

TIMP1

Synonyms:

Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA, Fibroblast collagenase inhibitor, Tissue Inhibitor of Metalloproteinase 1, TIMP

Species:

Human

Cat. No.:

CMT150 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$770.00 BUY

Description:

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Uniprot Name:

Metalloproteinase inhibitor 1

Uniprot ID:

P01033

Data PDF:

CMT150

Specificity:

Recognizes human TIMP1

Host Species:

Mouse

Immunogen:

Recombinant human TIMP1

Isotype:

IgG1

Clone:

4A16

Formulation:

Lyophilized from sterile filtered PBS

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Add 500 µl sterile PBS to the vial to fully solubilize the antibody to a concentration of 200 µg/ml.

Application:

IHC, WB

Application notes:

Western Blot: use a working dilution of 1:100-1000

Immunohistochemistry (Paraffin) : use a working dilution of 1:50-200.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Lyophilized antibody is stable for 1 year at -20°C to -80°C. Reconstituted antibody is stable for six months in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.