Mouse Anti-Human Tissue Factor Pathway Inhibitor Clone CLB/TFPI Kunitz-2 mAb

Molecule:

TFPI

Synonyms:

LACI, EPI, TFPI

Species:

Human

Cat. No.:

CS-MW1845 pdf (datasheet)

Quantity/Size:

1 mg

Description:

Mouse monoclonal antibody to Human Tissue Factor Pathway Inhibitor (TFPI). TFPI is a protease inhibitor with three tandem Kunitz-type inhibitor domains that inhibits the tissue factor (TF)-initiated blood coagulation cascade reactions by forming a complex with factor VIIa/TF via the first Kunitz-type inhibitor domain and with factor Xa via the second Kunitz-type inhibitor domain. The third Kunitz-type inhibitor domain of TFPI has no ability to inhibit protease but plays an important role in the functions of TFPI.

Entrez Symbol:

TFPI

Uniprot Name:

Tissue factor pathway inhibitor

Uniprot ID:

P10646

Specificity:

Kunitz domain 2 (aa 88-160)

Host Species:

Mouse

Isotype:

IgG1

Clone:

CLB/TFPI Kunitz-2

Formulation:

20 mM Tris and 150 mM NaCl, pH 8.0. Carrier and preservative free.

Purification:

Protein A affinity chromatography.

Application:

ELISA, FC, Neutralizing , WB

Application notes:

ELISA: Useful as coating antibody in human TFPI ELISA in conjunction with tracer antibody TFPI (Kunitz-1) (CS-MW1846) for tests in plasma and serum. This combination measures truncated and full-length TFPI.
The combination of TFPI (Kunitz-2) with TFPI (Kunitz-3)
(CS-MW1847) or with TFPI (C-terminus) (CS-MW1848) measures only full-length TFPI.
Western Blot, Flow Cytometry: This antibody is also useful for western blot analysis and in FACS analysis for detection of TFPI on endothelial and smooth muscle cells.
Neutralization studies: Neutralizes TFPI inhibition of TF/VIIa-Xa assay and Xa in chromgen assays. It also neutralizes TFPI inhibition (partly) in a diluted protrombin test.
The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.