Mouse Anti-Human TRAIL / APO2L (Clone B-T24) Biotin mAb

Synonyms:

Tumor necrosis factor ligand superfamily member 10, TNFSF10, Apo-2L, APO-2 ligand, CD253, TRAIL

Species:

Human

Cat. No.:

CDM439 pdf (datasheet)

Quantity/Size:

100 µg

Price:

$650.00 BUY

Concentration:

0.1 mg / 1.0 ml

Description:

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Symbol:

TNFSF10

Uniprot Name:

Tumor necrosis factor ligand superfamily member 10

Uniprot ID:

P50591

Data PDF:

CDM439

Specificity:

Recognizes Human TRAIL, APO2L antigen, a 32 kDa protein

Host Species:

Mouse

Immunogen:

Recombinant human TRAIL

Isotype:

IgG1

Clone:

B-T24

Conjugate:

Biotin

Formulation:

PBS containing 1% BSA and 0.09% Sodium Azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.

Purification:

Ion exchange chromatography

Application:

ELISA

Application notes:

ELISA Detection Antibody. This antibody can be used as a Detection Antibody (0.05-0.5 µg/ml) in a human TRAIL sandwich immunoassay to detect human TRAIL in combination with human TRAIL Capture Antibody (Cat No CDM415). The suggested coating concentration range below should be optimized by each laboratory for each application.

Storage/Stability:

Store at 2-8°C for 12 months. For longer storage, freeze aliquots at -20°C. Avoid repeated freeze-thaw cycles.