Mouse Anti-Human VEGFR1 / FLT1 (Clone EWF) mAb, Biotin

Molecule:

VEGFR1/FLT1

Synonyms:

Vascular Endothelial Growth Factor Receptor 1, FLT, fms-like tyrosine kinase 1, fmsrelated tyrosine kinase 1, Vascular Endothelial Growth Factor Receptor, VEGFC receptor

Species:

Human

Cat. No.:

CMV118 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$535.00 BUY

Description:

Recombinant Human Soluble Vascular Endothelial Growth Factor Receptor-1 (sVEGFR -1) is the naturally occurring form and was cloned from total RNA of human umbilical vein endothelial cells. The mature rh-sVEGFR-1 is a glycosylated monomeric protein with a mass of approximately 96kDa. The soluble receptor consists of the first 6 extracellular domains containing the unique 31 amino acids residues at the C-terminus. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.

The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis binding VEGF with the same affinity as the fulllength receptor.

Uniprot Name:

Vascular endothelial growth factor receptor 1

Uniprot ID:

P17948-2

Data PDF:

CMV118

Specificity:

The unconjugated antibody will detect native human VEGFR-1/Flt-1 in ELISA experiments and on the surface of different human cell types.

Host Species:

Mouse

Immunogen:

Recombinant human soluble extracellular Flt-1 (D5)

Isotype:

IgG1

Clone:

EWF

Conjugate:

Biotin

Formulation:

Lyophilized in a buffer of PBS, 50x BSA, 0.02% sodium azide.

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1-1.0 mg/ml.

Application:

ELISA, IP , WB

Application notes:

Western Blot: Use at 2-5 μg/ml
ELISA: Use at 1-10 μg/ml
Immunoprecipitation: Use at 2-10 μg/ml
The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.