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Mouse Anti-Human VEGF Clone 3 (6D3) mAb

Molecule:

VEGF

Synonyms:

vascular permeability factor, VPF, VEGF, MVCD1

Species:

Human

Cat. No.:

CMV015 pdf (datasheet)

Quantity/Size:

200 μg

Price:

$695.00 BUY

Description:

The mouse monoclonal antibody recognizes human Vascular Endothelial Growth Factor (VEGF)-A, a member of the PDGF/VEGF growth factor family. VEGF-A is often found as a disulfide linked homodimer. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Two high-affinity tyrosine kinase receptors for VEGF exist, VEGFR-1/FLT-1 and VEGFR-2/KDR.

Entrez Symbol:

VEGFA

Uniprot Name:

Vascular endothelial growth factor A

Uniprot ID:

P15692

Data PDF:

CMV015

Specificity:

Human

Host Species:

Mouse

Immunogen:

Recombinant human VEGF165 protein (45 kDa).

Isotype:

IgG1

Clone:

3 (6D3)

Formulation:

Lyophilized from PBS, pH 7.4 without preservative and additives.

Purification:

Protein G Chromatography

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1 to 1.0 mg/mL.

Application notes:

ELISA: Use at 1 - 15 g/ml | Neutralization: Block the VEGF-A induced proliferation of ACE cell at a 1:6 molar ratio of ligand to antibody. Inhibit the binding of VEGF-A to the VEGF receptors 1 (Flt-1) and 2 (KDR) | Western blotting: Use at 2-10 g/ml. Op

Storage/Stability:

Product is stable upon receipt for one month at RT. After reconstitution, antibody is stable for two weeks at 2-8 °C or for six months in working aliquots at -20 °C. Avoid repeated freeze-thaw cycles.