Mouse Anti-Mouse CD14 Clone biG 53 mAb

Molecule:

CD14

Synonyms:

Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein

Species:

Mouse

Cat. No.:

CMC005 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$540.00 BUY

Description:

Clone biG 53 represents an excellent marker for mouse CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage-like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore, CD14 is present in a soluble form in mouse serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Uniprot Name:

Monocyte differentiation antigen CD14

Uniprot ID:

P10810

Data PDF:

CMC005

Host Species:

Mouse

Immunogen:

Recombinant mouse CD14

Isotype:

IgG2a

Clone:

biG 53

Formulation:

Lyophilized from a 1.0 mg/ml solution in PBS without NaN3.

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Reconstitute with 100 µl sterile, distilled water and prepare working aliquots. Further dilution should contain 0.1% BSA for stability and to prevent loss.

Application notes:

Suitable for immunostaining of CD14 positive cells, inhibition studies, and ELISA. Binds LPS-activated mouse monocytes, mouse CD14-transfected cells, or recombinant mouse CD14.

Inhibits binding of LPS to CD14. Up to 1 µg/ml biG 53 inhibits binding of LPS (0.5 µg/ml) to 6 x 105 CD14-transfected CHO cells as determined by Flow Cytometry. Binding titer of mouse CD14 transfected CHO cells: > 1:20,000 (ELISA, Flow cytometry). The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store working aliquots at -20°C to -80°C for up to 1 year.