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Mouse Anti-RFP Tag (Clone RF5R) mAb

Cat. No.:

CSI20570A pdf (datasheet)

Quantity/Size:

50 μg

Price:

$165.00 BUY

Description:

Mouse Anti-RFP (Red Fluorescent Protein) Tag Clone RF5R mAb

Uniprot Name:

Red fluorescent protein drFP583

Uniprot ID:

Q9U6Y8

Data PDF:

CSI20570

Specificity:

Recognizes native and denatured forms of RFP and its variants: Tag-RFP, Turbo-RFP, dsRed, mCherry, mOrange, tdTomato, etc.

Host Species:

Mouse

Immunogen:

N-terminal peptide from Discosoma sp. (sea anemone) Red Fluorescent Protein, peptide conjugated to KLH (Keyhole Limpet Hemocyanin).

Isotype:

IgG1

Clone:

RF5R

Conjugate:

Unconjugated

Formulation:

Liquid in 10 mM PBS, pH 7.2 with 0.05% sodium azide

Purification:

Protein A affinity chromatography from mouse ascites fluid

Application:

DB , ELISA, IP , IS , WB

Application notes:

Western blot sensitivity (with ECL) : 1-10 ng of purified RFP or RFP fusion proteins. Recommended working dilutions:

Western Blot (with ECL) ; 1:1,000-3,000 (incubate for one hour at room temperature).

Immunoprecipitation: 1:50-200.

Immunostaining: 1:500-2,000.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store at 2-8°C for up to two weeks, or in working aliquots at -20°C to -80°C for up to one year. Avoid repeated freeze-thaw cycles.