Mouse Cerebral Cortex Tissue Lysate

Species:

Mouse

Cat. No.:

CS378B pdf (datasheet)

Quantity/Size:

400 μg

Price:

$335.00 BUY

Description:

A freshly-harvested Balb C mouse brain is dissected and the cerebral cortex is flashfrozen in liquid nitrogen and stored frozen until lysis. The tissue is ground and thoroughly homogenized at 4C with a freshly prepared modified Radio Immuno Precipitation Assay (RIPA) buffer containing strong protease and phosphatase inhibitor cocktails. The final homogenate is incubated with DNase then clarified by centrifugation at 10,000 g at 4C and stored at -80C. Each lot is quality controlled by determining the protein concentration using a BCA assay and analyzed with SDS-PAGE using Coomassie blue for visualization. Sharp, clear protein bands indicate a quality protein lysate.

Concentration:

2.5 mg/ml

Data PDF:

CS378

Formulation:

Liquid in 0.1 M Tris-HCl, 0.15 M NaCl, 0.1% SDS, 0.5% Na Deoxycholate, 1% Triton X -100, pH 7.4

Storage/Stability:

Store at -80°C for up to 1 year. Upon initial thaw, prepare working aliquots and store at -80°C.