Mouse Colon Tissue Lysate

Species:

Mouse

Cat. No.:

PX370A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$190.00 BUY

Description:

Mouse colon tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl + pH 7.4 + 1% Triton X-100 + 0.2% sodium deoxycholate + 0.2% sodium dodecylsulfate (SDS) + 1 mM sodium ethylenediaminetetraacetate + 1 mM phenylmethylsulfonyl fluoride + 5 µg/ml of aprotinin + 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl, pH 6.8 + 12.5% glycerol + 1% SDS + 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Concentration:

2.0 mg/ml

Data PDF:

PX370

Formulation:

Colon issue lysate in 1 x SDS sample buffer containing 5% β-mercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. Mouse colon tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

Application:

WB