The Mouse MPO (Myeloperoxidase) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MPO in plasma (serum is not recommended in this assay due to mouse MPO being released from neutrophils into serum in the process of blood coagulation) and cell culture supernatants. This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Included in the kit:
Microplate coated with Anti-Mouse MPO
Wash Buffer Concentrate (20x)
Recombinant Mouse MPO Standards
Assay Diluent C: Standard/Sample - Plasma Diluent Buffer
Assay Diluent B (5x): Standard/Sample - Cell Culture Medium Diluent
Detection Antibody: Anti-Mouse MPO Biotin
Streptavidin-HRP Concentrate
TMB One-Step Substrate Reagent (3, 3’, 5, 5’-tetramethylbenzidine in buffered solution)
