Mouse TNF-alpha ELISA Kit

Molecule:

TNF-alpha

Species:

Mouse

Cat. No.:

CKM064 pdf (datasheet)

Quantity/Size:

1 Plate

Price:

$575.00 BUY

Description:

The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Mouse TNF-α coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-α present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse TNF-α antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Data PDF:

CKM064