Mouse VEGF ELISA Kit

Molecule:

VEGF

Synonyms:

Vascular endothelial growth factor, VEGF, VEGF-A

Cat. No.:

CKM066 pdf (datasheet)

Quantity/Size:

1 Plate

Price:

$575.00 BUY

Description:

The Cell Sciences® Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse VEGF coated on a 96- well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Data PDF:

CKM066