Mouse IFN-gamma ELISPOT Kit w/ plates

Molecule:

IFN-gamma

Synonyms:

Ifg, IFN-g, IFN-gamma, gamma interferon

Species:

Mouse

Cat. No.:

CKM038S pdf (datasheet)

Quantity/Size:

5 x 96-well PVDF plates

Price:

$1,395.00 BUY

Description:

The Mouse IFN-gamma ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect Mouse IFN-gamma release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA or intracellular staining is due to the plate-bound antibodies directly capturing the Mouse IFN-gamma released by the cell before it is diluted in the supernatant, trapped by high-affinity receptors or degraded by proteases. The sensitivity of the assay lends itself to measurement of very low frequency of Mouse IFN-gamma-secreting cells (1/300,000).

Entrez Symbol:

Ifng

Uniprot Name:

Interferon gamma

Uniprot ID:

P01580

Kit Plates:

PVDF plates

Storage/Stability:

Store all reagents at 2-8°C.