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The mouse LBP kit is a solid phase sandwich Enzyme Linked-Immunosorbent Assay (ELISA) developed for the quantitative measurement of natural and recombinant mouse LBP (both free and LPS-bound) in serum, plasma and culture medium. This kit is also useful for detection of rat LBP.
Normal LBP range in untreated mice: 2-15 µg/mL. (Acute phase sera may contain a factor of 10 to 100 fold more LBP.)
Interassay CV: 7% to 13.6% depending on the concentration
Intrassay CV: 2.4%, n = 50 plasma samples
Effective range: 3 - 50 ng/mL
Cross-reactivity: rat LBP
Specificity: detects free and bound LBP
Recovery of recombinant LBP in LBP-depleted sera is 100%.