Native Bovine Alkaline Phosphatase, Intestinal-type

Molecule:

Alkaline Phosphatase/ALPI

Synonyms:

IAP, intestinal alkaline phosphatase, intestinal alkaline phosphatase V

Species:

Bovine

Cat. No.:

CSI14880 pdf (datasheet)

Quantity/Size:

5 mg

Price:

$370.00 BUY

Description:

The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.

Entrez Symbol:

ALPI

Uniprot ID:

P19111

Data PDF:

CSI14879

Molecular Weight:

140 kDa

Physical State:

Sterile filtered (0.2 micron) liquid.

Formulation:

50% glycerol, 5 mM MgCl2, 0.1 mM ZnCl2 and 5 mM Tris-HCl, pH 7.0.

Purity:

95% pure by Gel Filtration.

Biological Activity:

>1500 U/mg at pH 9.6, 25°C, 0.025 M glycine, with 10% glycerol buffer.

Storage/Stability:

Store at 2-8°C. Do not freeze.