Avidin, Egg White

Molecule:

Avidin

Cat. No.:

CSI20289B pdf (datasheet)

Quantity/Size:

500 mg

Price:

$970.00 BUY

Description:

Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including western blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.

CAS No:

1405-69-2

Data PDF:

CSI20289

Source:

Avian Egg White

Molecular Weight:

66 kDa with subunits at 16 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized

Biological Activity:

> 10 U/mg powder

Specific Activity:

>10 U/mg powder

Application notes:

Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including western blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucl