Native Human IGFBP1

Molecule:

IGFBP1

Synonyms:

IGFBP-1, IBP-1 Placental protein 12, PP12

Species:

Human

Cat. No.:

BP1BU020 pdf (datasheet)

Quantity/Size:

20 μg

Price:

$460.00 BUY

Description:

Human Insulin-like growth factor binding protein-1 (IGFBP-1) is a 234 amino acid protein which is a member of a family of six circulating proteins that bind IGF-I and IGF-II with high affinity and modulate their metabolic and mitogenic effects. The liver, ovarian granulosa cells, decidualized endometrium and a number of other cell types produce native human IGFBP-1. IGFBP-1 binds both IGF-I and IGF-II. Serum IGFBP-1 levels are regulated by insulin-induced inhibition of IGFBP-1 production. IGFBP-1 appears to inhibit smooth muscle migration in response to IGF-I or IGF-II and to stimulate wound healing in response to IGF-II. Native IGFBP-1 is predominantly a lower molecular weight species of 218 amino acids and 3-4 phosphorylated forms.

Uniprot Name:

Insulin-like growth factor-binding protein 1

Uniprot ID:

P08833

Data PDF:

BP1BU020

Molecular Weight:

25.3 kDa

Formulation:

Lyophilized from 10 mM HCl, sealed under dry nitrogen at a slight vacuum.

Purity:

> 95% by FPLC and SDS-PAGE

Biological Activity:

Binds both IGF-I and IGF-II
Inhibition of IGF-I stimulated proliferation of NUC-1 cells: IC50 ~ 100 ng/ml.
Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures: IC50 ~ 300 ng/ml.

Endotoxin Level:

< 0.1 EU/µg

Storage/Stability:

Store as supplied for up to 1 year at 2-8°C.

Application notes:

Protocol 3003 Procedure for Western Ligand blotting using iodinated IGF-I or IGF-II
Protocol 3004 Procedure for Western Ligand blotting using biotinylated IGF-I or IGF-II