Synonyms:
IGFBP-1, IBP-1 Placental protein 12, PP12
Description:
Human Insulin-like growth factor binding protein-1 (IGFBP-1) is a 234 amino acid protein
which is a member of a family of six circulating proteins that bind IGF-I and IGF-II with
high affinity and modulate their metabolic and mitogenic effects. The liver, ovarian
granulosa cells, decidualized endometrium and a number of other cell types produce
native human IGFBP-1. IGFBP-1 binds both IGF-I and IGF-II. Serum IGFBP-1 levels are
regulated by insulin-induced inhibition of IGFBP-1 production. IGFBP-1 appears to inhibit
smooth muscle migration in response to IGF-I or IGF-II and to stimulate wound healing in
response to IGF-II. Native IGFBP-1 is predominantly a lower molecular weight species of
218 amino acids and 3-4 phosphorylated forms.
Uniprot Name:
Insulin-like growth factor-binding protein 1
Molecular Weight:
25.3 kDa
Formulation:
Lyophilized from 10 mM HCl, sealed under dry nitrogen at a slight vacuum.
Purity:
> 95% by FPLC and SDS-PAGE
Biological Activity:
Binds both IGF-I and IGF-II
Inhibition of IGF-I stimulated proliferation of NUC-1 cells: IC50 ~ 100 ng/ml.
Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures: IC50 ~ 300 ng/ml.
Endotoxin Level:
< 0.1 EU/µg
Storage/Stability:
Store as supplied for up to 1 year at 2-8°C.
Application notes:
Protocol 3003 Procedure for Western Ligand blotting using iodinated IGF-I or IGF-II
Protocol 3004 Procedure for Western Ligand blotting using biotinylated IGF-I or IGF-II
