Recombinant Human Serpin E1/PAI-1 Stable NBD-labeled Scissor Bond Point Mutant

Synonyms:

Plasminogen Activator Inhibitor type 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor

Cat. No.:

CSI20512B pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,935.00 BUY

Description:

Recombinant Human Serpin E1/PAI-1 Scissor Bond NBD-labeled Stable Point Mutant is a fluorescence-labeled probe produced by the mutagenesis of the P1' methionine residue (Met347) at the P1-P1' scissile bond of the Human Serpin E1/PAI-1 stable mutant (K154T, Q319L, M354I and N150H) to cysteine. Subsequent incorporation of the thiol-reactive iodoacetamide dye NBD [N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] produced a reporter PAI-1 with extended half life.

This reagent is useful as a tracer probe because its fluorescence is quenched as the as the PAI-1 structure is changed by protein-protein interactions. Other properties of the NBD-PAI-1 mutant have not been fully characterized.

Entrez Symbol:

SERPINE1

Uniprot Name:

Plasminogen activator inhibitor 1

Uniprot ID:

P05121

Data PDF:

CSI20512

Source:

E. coli

Molecular Weight:

43 kDa

Physical State:

Liquid

Formulation:

Liquid in 0.05 M Sodium Phosphate, 0.1 M NaCl, 1 mM EDTA, pH 6.6

Endotoxin Level:

< 0.1 ng/µg of protein.

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.