Native Porcine ECGF

Molecule:

ECGF

Synonyms:

Endothelial cell growth factor, ECGF

Species:

Porcine

Cat. No.:

CRE127 pdf (datasheet)

Quantity/Size:

6 mg

Price:

$205.00 BUY

Description:

Endothelial cell growth factor (ECGF) is an extract of porcine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cells from human umbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows to cultivate endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.

Data PDF:

CRE127

Source:

Pig

Formulation:

Lyophilized from sterile-filtered solution containing NaCl and streptomycin sulfate.

Biological Activity:

Optimum concentration for human umbilical vein endothelial cells (HUVEC) with heparin (50 μg/ml) is at 10-20 μg/ml. As a growth supplement for use in monoclonal antibody production the optimum range is 50 to 200 μg/ml.

Storage/Stability:

Store as supplied at -20°C to -80°C.

Application notes:

Porcine ECGF is effective on mouse, bovine, porcine and human cells. ECGF is a crude extract. The solution mayy not be not fully clear after reconstitution. However this has no influence on the activity!