Porcine ECGF, Crude Extract for Cell Culture

Molecule:

ECGF

Synonyms:

Endothelial Cell Growth Factor

Species:

Porcine

Cat. No.:

CSI11673 pdf (datasheet)

Quantity/Size:

6 mg

Price:

$200.00 BUY

Description:

Porcine Endothelial Cell Growth Factor (ECGF), Crude Extract for Cell Culture is an extract of pig brain containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGF has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Endothelial cells from human umbilical vein (HUVEC) can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows to cultivate endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types. This product does not contain Heparin. See CRE127 for Porcine ECGF, Crude Extract for Cell Culture with Heparin

Data PDF:

CSI11673

Source:

Pig

Formulation:

Lyophilized from PBS, pH 7.4 without preservatives.

Purity:

Crude extract

Biological Activity:

The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal.

Storage/Stability:

Store at 2-8°C for 12 months.

Application notes:

Porcine ECGF is effective on mouse, bovine, pig and human cells.