Native Porcine Pancreatic Elastase

Synonyms:

Chymotrypsin-like elastase family member 1, Elastase-1

Species:

Porcine

Cat. No.:

CRP262C pdf (datasheet)

Quantity/Size:

20 mg

Price:

$605.00 BUY

Description:

Porcine Pancreatic Elastase was chromatographically prepared from the euglobin fraction of porcine pancreas by the method of Lewis et al. then twice crystallized. Supplied as a salt free lyophilized powder that is free of trypsin and chymotrypsin. Lewis, U.J. et al. (1956) J. Biol. Chem. 22:705

Entrez Symbol:

CELA1

Uniprot Name:

Chymotrypsin-like elastase family member 1

Uniprot ID:

P00772

Data PDF:

CRP262

Molecular Weight:

25.2 kDa

Physical State:

Lyophilized

Purity:

Homogenous on 15% polyacrylamide electrophoresis at pH 4.5 and on gel exclusion chromatography (G200) at pH 5.0 and 25°C. Trypsin not detected on 0.5 mg elastase after more than 24 hour incubation at 25°C on the substrate N-benzoyl-DL-arginine-pNA (BAPNA)

Biological Activity:

Assay: 5-10 mg in 0.1M Tris pH 8.8 at 37°C. containing 0.2mM Suc-(Al)3-pNA.

Specific Activity:

> 9 units/mg of protein on the substrate Suc-Ala-Ala-Ala-pNA. One unit will hydrolyze 1 µmole per minute at pH 8.3 and 25°C.

Reconstitution:

Reconstitute with 0.05M Na Acetate, 0.1M NaCl pH 5.0 to 1 mg/ml or desired concentration, aliquot and store at -20°C to -80°C.

Storage/Stability:

Stable lyophilized for 1 year at 2-8°C.