Rabbit Anti-Human Caspase-3 (Active) Affinity Purified pAb

Synonyms:

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1

Species:

Human

Cat. No.:

CPC109 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$880.00 BUY

Concentration:

0.5 mg/ml

Description:

Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase 3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase 3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).
This affinity purified antibody was prepared from rabbits immunized with a synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3 and recognizes the p17 fragment of the active caspase-3 to provide a tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Entrez Symbol:

CASP3

Uniprot Name:

Caspase-3

Uniprot ID:

P42574

Data PDF:

CPC109

Specificity:

Recognizes the p17 fragment of the active caspase-3

Host Species:

Rabbit

Immunogen:

Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3

Formulation:

PBS, pH 7.2 containing 30% glycerol, 0.5% BSA, 5 mM EDTA, 0.03% Proclin.

Purification:

Antigen affinity chromatography

Application:

IHC, WB

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.