Rabbit Anti-Human IGF-1 Biotin pAb

Molecule:

IGF-I

Synonyms:

Erythropoietic factor, Somatomedin C, IGF-I, Mechano growth factor, MGF

Species:

Human

Cat. No.:

PA0362BT pdf (datasheet)

Quantity/Size:

50 μg

Price:

$590.00 BUY

Description:

Insulin-like Growth Factor 1 (IGF1) is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. IGF1 is processed from a precursor, bound by a specific receptor, and secreted. Defects in IGF1 gene are a cause of Insulin-like Growth Factor 1 deficiency.

Entrez Symbol:

IGF1

Uniprot Name:

Insulin-like growth factor I

Uniprot ID:

P05019-3

Data PDF:

PA0362BT

Specificity:

Human IGF1

Host Species:

Rabbit

Immunogen:

Highly purified (>98%) Recombinant Human IGF1, Isoform 3

Conjugate:

Biotin

Formulation:

Lyophilized from sterile filtered PBS, pH 7.2

Purification:

Immobilized hIGF-1 affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Add sterile PBS containing 0.1% BSA to fully solubilize the antibody to a concentration of 0.1-1.0 mg/mL.

Application:

ELISA, WB

Application notes:

Sandwich ELISA: To detect hIGF-I by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction with PA0362, polyclonal anti-human IGF-1, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-I.

Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store at -20°C to -80°C for up to 1 year. After reconstitution, stable for two weeks at 2-8° C. It is recommended to store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.