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Sandwich ELISA: To detect hIGF-I by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction with PA0362, polyclonal anti-human IGF-1, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-I.
Western Blot: To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
The optimal concentration should be determined by the user for each specific application.