Rabbit Anti-Human IGF-IIE (aa 78-88) pAb

Molecule:

IGF2

Species:

Human

Cat. No.:

PAAZ1 pdf (datasheet)

Quantity/Size:

100 μl

Price:

$380.00 BUY

Description:

Human ProIGF-II E (78-88) antiserum was prepared by immunizing rabbits with an 11 amino acid peptide derived from the E domain of human IGF-II E linked to diphtheria toxoid. The sequence corresponds to IGF-II E77-88and as a consequence, the antiserum recognizes full length IGF-II E and both IGF-II E variants (IGF-II E1-88 andIGF-II E1-104) derived from processing of the human igf-2 gene and following proteolytic cleavage. It does not recognize mature IGF-II or IGF-I. Sequence analysis suggests it is likely to recognize IGF-II E proteins in non-human primates, dog, guinea-pig, pig and a number of other species.

Uniprot Name:

Isoform 1

Uniprot ID:

P01344-1

Data PDF:

PAAZ1

Specificity:

Determined by Western blotting. Recognizes full length human IGFIIE (IGF-IIE1-156) and proteolytically cleaved variants (IGF-IIE -87) and (IGF-IIE1-104).

Host Species:

Rabbit

Immunogen:

Derived from UniProt ID P03144 isoform 1. The peptide sequence is amino-acids 102-112, derived from IGF-IIE, conjugated to diphtheria toxoid.

Formulation:

Lyophilized antiserum

Reconstitution:

Centrifuge vial prior to opening. Dissolve in 100 µl sterile water.. Dilute in a buffer suitable for the desired application.

Application:

WB

Application notes:

Western Blotting: 1:4,000
Enzyme Immunoassay: 1:5,000
The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Lyophilized product is stable for at least one year at 2-8°C. After reconstitution, the contents can be safely stored for up to 1 year in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.