Rabbit Anti-Human MAP3K14, NIK pAb

Molecule:

NIK

Synonyms:

Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK, Serine/threonine-protein kinase NIK

Species:

Human

Cat. No.:

CPN000 pdf (datasheet)

Quantity/Size:

200 μg

Price:

$260.00 BUY

Description:

NIK (NF-kB-inducing kinase) is a member of the MAP kinase family that binds TRAF2 and stimulates NF-kB activity. NIK, initially isolated from a human B cell cDNA library, contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1 induced NFkB activation.

Entrez Symbol:

MAP3K14

Uniprot Name:

Mitogen-activated protein kinase kinase kinase 14

Uniprot ID:

Q99558

Data PDF:

CPN000

Specificity:

Recognizes both natural and recombinant Human NIK

Host Species:

Rabbit

Immunogen:

Recombinant Human NIK fragment (aa 795-947), expressed in E. coli.

Isotype:

IgG

Conjugate:

Unconjugated

Formulation:

PBS with 0.1% sodium azide

Purification:

Protein A affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Add 200 µL sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1.0 mg/mL.

Application:

ELISA, IP , WB

Application notes:

Western blot: working dilution of 1:2,000
Immunoprecipitation: working dilution of 1:500

Storage/Stability:

Store at 2-8 °C for 1 week. Store working aliquots at -20°C to -80°C for up to 1 year.