Rabbit Anti-Rat MCP-1 pAb

Molecule:

CCL2

Synonyms:

Monocyte chemotactic protein 1, MCP-1, C-C motif chemokine 2, CCL2, Small inducible cytokine A2, Immediate-early serum-responsive protein JE

Species:

Rat

Cat. No.:

CPM000 pdf (datasheet)

Quantity/Size:

0.5 mg

Price:

$280.00 BUY

Description:

Chemokine (C-C motif) Ligand 2, also known as Monocyte Chemoattractant Protein 1 (MCP-1). Rat CCL2/MCP1 is a 148-amino acid C-C chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con Astimulated rat spleen cDNA library. This rat MCP-1 is 49 amino acids longer than human MCP-1 at 3’-end. This 3’-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains the higher molecular weight (25 kDa). In vitro, MCP-1 is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1 is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.

Entrez Symbol:

Ccl2

Uniprot Name:

CCL2_RAT

Uniprot ID:

P14844

Data PDF:

CPM000

Specificity:

Rat CCL2 / MCP-1

Host Species:

Rabbit

Immunogen:

Recombinant Rat CCL2 / MCP-1

Isotype:

IgG

Conjugate:

Unconjugated

Formulation:

Lyophilized with 0.1% sodium azide.

Purification:

Protein A affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Add 500 µL sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1 mg/mL.

Application:

IHC, NT, WB

Application notes:

Western blot: 1:2000
Immunohistochemistry: 1:200

Storage/Stability:

Store at 2-8°C for up to one month or in working aliquots at -20°C for up to one year. Avoid repeated freeze-thaw cycles.